- PA5-49916 - Invitrogen Antibodies COL14A1 antibody

Submitted references Conditional deletion of Nedd4-2 in lung epithelial cells causes progressive pulmonary fibrosis in adult mice.

Duerr J, Leitz DHW, Szczygiel M, Dvornikov D, Fraumann SG, Kreutz C, Zadora PK, Seyhan Agircan A, Konietzke P, Engelmann TA, Hegermann J, Mulugeta S, Kawabe H, Knudsen L, Ochs M, Rotin D, Muley T, Kreuter M, Herth FJF, Wielpütz MO, Beers MF, Klingmüller U, Mall MA
Nature communications 2020 Apr 24;11(1):2012

Regulators of Collagen Fibrillogenesis during Molar Development in the Mouse.

Zvackova I, Matalova E, Lesot H
Frontiers in physiology 2017;8:554

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of COL14A1 in HeLa cells. Samples were probed using a COL14A1 polyclonal antibody (Product # PA5-49916).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemical analysis of COL14A1 in HepG2 cells. The cells were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The cells were incubated with COL14A1 Polyclonal Antibody (Product # PA5-49916) at a 1:200 dilution for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1:600, was used as the secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Localization of FACITs and SLRPs in the developing tooth and surrounding structures at embryonic day 18 (E18) and perinatal day (P0). (A) Haematoxylin and eosin staining of embryonic tooth and surrounding tissues (E18). Details from boxes (B-D) in A is shown in B,C , and Figure 2D . (B) Detail of dental pulp. (C) Detail of peridental mesenchyme and alveolar bone. Expression of biglycan in dental (B1) and peridental mesenchymes (C1) . Expression of collagen XIIa in dental (B2) and peridental mesenchymes (C2) . Expression of collagen XIVa in dental (B3) and peridental mesenchymes (C3) . Expression of decorin in dental (B4) and peridental mesenchymes (C4) . Expression of fibromodulin in dental (B5) and peridental mesenchymes (C5) . (E) Haematoxylin and eosin staining of the tooth and surrounding tissues at P0. Details from boxes (F-H) in E is shown in F , G , and Figure 2H . (F) Detail of dental pulp and predentin. (G) Detail of periodontal ligaments and alveolar bone. Expression of biglycan (F1) , collagen XIIa (F2) , collagen XIVa (F3) , decorin (F4) , and fibromodulin (F5) in predentin and dental pulp. Expression of biglycan (G1) , collagen XIIa (G2) , collagen XIVa (G3) , decorin (G4) , and fibromodulin (G5) in the periodontal ligament and alveolar bone. Dp, dental pulp; pm, peridental mesenchyme; ab, alveolar bone; pd, predentin; pdl, periodontal ligaments; BGN, biglycan; COL12, collagen type XIIa; COL14, collagen type XIVa; DCN, decorin; FMOD; fibromodulin; HE, ha

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Localization of FACITs and SLRPs in predentin, dentin, periodontal ligaments, and alveolar bone at P11. Haematoxylin eosin staining of predentin/dentin and PDL in the crown (E) and root portions (G) . Haematoxylin eosin staining of PDL and alveolar bone in the crown portion (F) and root portion (H) . Expression of biglycan in predentin/dentin (E1) , PDL (E1,F1) and alveolar bone (F1) in the crown portion; predentin/dentin/cementum (G1) , PDL (G1,H1) and alveolar bone (H1) in the root portion. Expression of collagen XIIa in predentin/dentin (E2) , PDL (E2,F2) and alveolar bone (F2) in the crown portion; predentin/dentin/cementum (G2) , PDL (G2,H2) and alveolar bone (H2) in the root portion. Expression of collagen XIVa in predentin/dentin (E3) , PDL (E3,F3) and alveolar bone (F3) in the crown portion; predentin/dentin/cementum (G3) , PDL (G3,H3) and alveolar bone (H3) in the root portion. Expression of decorin in predentin/dentin (E4) , PDL (E4,F4) and alveolar bone (F4) in the crown portion; predentin/dentin/cementum (G4) , PDL (G4,H4) and alveolar bone (H4) in the root portion. Expression of fibromodulin in predentin/dentin (E5) , PDL (E5,F5) and alveolar bone (F5) in the crown portion; predentin/dentin/cementum (G5) , PDL (G5,H5) and alveolar bone (H5) in the root portion. Pd, Predentin; d, dentin; pdl, Periodontal ligaments; ab, alveolar bone; ce, cementum; BGN, biglycan; COL12, collagen type XIIa; COL14, collagen type XIVa; DCN, decorin; FMOD; fibromodulin; HE, ha

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Localization of FACITs and SLRPs in predentin/dentin, periodontal ligaments and alveolar bone at P22. Haematoxylin eosin staining of dentin and PDL in the crown area (K) and predentin/dentin, PDL and cementum in the root portion (M) . Haematoxylin eosin staining of PDL and alveolar bone in the crown (L) and root portions (N) . Expression of biglycan in dentin (K1) , PDL (K1,L1 ) and alveolar bone (L1) in the crown portion; predentin/dentin/cementum (M1) , PDL (M1,N1) and alveolar bone (N1) in the root portion. Expression of collagen XIIa in dentin (K2) , PDL (K2,L2) and alveolar bone (L2) in the crown portion; predentin/dentin/cementum (M2) , PDL (M2,N2) and alveolar bone (N2) in the root portion. Expression of collagen XIVa in dentin (K3) , PDL (K3,L3) and alveolar bone (L3) in the crown portion; predentin/dentin/cementum (M3) , PDL (M3,N3) and alveolar bone (N3) in the root portion. Expression of decorin in dentin (K4) , PDL (K4, L4) and alveolar bone (L4) in the crown portion; predentin/dentin/cementum (M4) , PDL (M4,N4) and alveolar bone (N4) in the root portion. Expression of fibromodulin in dentin (K5) , PDL (K5,L5) , and alveolar bone (L5) in the crown portion; predentin/dentin/cementum (M5) , PDL (M5,N5) , and alveolar bone (N5) in the root portion. Pd, Predentin; d, dentin; pdl, periodontal ligaments; ab, alveolar bone; ce, cementum; BGN, biglycan; COL12, collagen type XIIa; COL14, collagen type XIVa; DCN, decorin; FMOD; fibromodulin; HE, haematoxylin/eosin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 Co-expression of FACITs and SLRPs in early alveolar bone development. (A) Haematoxylin and eosin staining of embryonic tooth and surrounding tissues at E14. (B) Detail of alveolar bone at E14. (C) Haematoxylin and eosin staining of embryonic tooth and surrounding tissues at E15. (D) Detail of alveolar bone at E15. Expression of biglycan (B1) , collagen XIIa (B2) , collagen XIVa (B3) , decorin (B4) , fibromodulin (B5) in developing alveolar bone at E14. Expression of biglycan (D1) , collagen XIIa (D2) , collagen XIVa (D3) , decorin (D4) , fibromodulin (D5) in developing alveolar bone at E15. Ab, Alveolar bone; BGN, biglycan; COL12, collagen type XIIa; COL14, collagen type XIVa; DCN, decorin; FMOD; fibromodulin; HE, haematoxylin/eosin. Scale bar = 25 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 7 Comparison of proteomic signatures of pulmonary fibrosis in conditional Nedd4-2 -/- mice and patients with IPF. a , b Heatmap of differentially regulated matrisome annotated proteins in lungs from conditional Nedd4-2 -/- mice ( n = 11) and control mice ( n = 13) induced with doxycycline for 3 months ( a ), and in lung tissues from IPF patients and controls ( n = 11/group) ( b ). c Venn-diagram showing proportion of unique and common differentially regulated proteins in lungs of conditional Nedd4-2 - /- mice and IPF patients. d Summary of differentially regulated matrisome proteins found in conditional Nedd4-2 - /- mice and human IPF samples. e Micrographs of representative lung sections from conditional Nedd4-2 - /- mice and littermate controls stained with anti-Tnc, anti-Col14a1, and anti-Serpinh1 antibodies ( n = 6/group). Scale bars, 100 um.

PA5-49916

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