PA5-18140

antibody from Invitrogen Antibodies

Targeting: PARK7 DJ-1, DJ1, GATD2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18140

Provider

Invitrogen Antibodies

Product name

DJ-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

100 μg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of DJ-1 using DJ-1 Polyclonal Antibody (Product # PA5-18140) (5 µg/mL) in MCF7 (A) and Hela (B) cells. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (PFA fixed) analysis of DJ-1 using DJ-1 Polyclonal Antibody (Product # PA5-18140) (7 µg/mL) in staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Negative Control for DJ-1 Polyclonal Antibody (Product # PA5-18140), showing immunohistochemical staining of paraffin embedded Human Testis, with no primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DJ-1 in human prostate. Samples were incubated with DJ-1 polyclonal antibody (Product # PA5-18140) using a dilution of 3.75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DJ-1 in human thyroid. Samples were incubated with DJ-1 polyclonal antibody (Product # PA5-18140) using a dilution of 3.75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.