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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- LS-C60118 - Provider product page
- Provider
- LSBio
- Product name
- WNT1 Antibody LS-C60118
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -20°C.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-Wnt1 Antibody - Western Blot. Western blot of affinity purified anti-Wnt1 antibody shows detection of endogenous Wnt1 in human-derived MCF7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2). The band at ~41 kD, indicated by the arrowhead, corresponds to Wnt1. After transfer, the membrane was blocked with 5% BLOTTO. Primary antibody was used at a 1:1400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt1 (data not shown).
