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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB11291 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11291, RRID:AB_1717100
- Product name
- WNT1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of WNT1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Suppressing Wnt signaling by the hedgehog pathway through sFRP-1.
A Wnt-Axin2-GSK3beta cascade regulates Snail1 activity in breast cancer cells.
The ups and downs of Wnt signaling in prevalent neurological disorders.
He J, Sheng T, Stelter AA, Li C, Zhang X, Sinha M, Luxon BA, Xie J
The Journal of biological chemistry 2006 Nov 24;281(47):35598-602
The Journal of biological chemistry 2006 Nov 24;281(47):35598-602
A Wnt-Axin2-GSK3beta cascade regulates Snail1 activity in breast cancer cells.
Yook JI, Li XY, Ota I, Hu C, Kim HS, Kim NH, Cha SY, Ryu JK, Choi YJ, Kim J, Fearon ER, Weiss SJ
Nature cell biology 2006 Dec;8(12):1398-406
Nature cell biology 2006 Dec;8(12):1398-406
The ups and downs of Wnt signaling in prevalent neurological disorders.
De Ferrari GV, Moon RT
Oncogene 2006 Dec 4;25(57):7545-53
Oncogene 2006 Dec 4;25(57):7545-53
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using WNT1 polyclonal antibody (Cat # PAB11291) shows detection of endogenous WNT1 in human-derived MCF-7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2).The band at ~41 kDa, indicated by the arrowhead, corresponds to WNT1.After transfer, the membrane was blocked with 5% BLOTTO.Primary antibody was used at a 1 : 1,400 dilution in PBS containing 1% BLOTTO.The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with WNT1 (data not shown).
