LS-C774840

antibody from LSBio

Targeting: WNT1 INT1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C774840

Provider

LSBio

Product name

WNT1 Antibody (Atto 633) LS-C774840

Antibody type

Polyclonal

Description

Peptide Affinity Purified

Reactivity

Human

Host

Rabbit

Conjugate

Red dye

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~41 kDa Wnt1 protein using Rabbit Anti-Wnt1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~41 kDa. Other Band(s): Band is detected at ~46 kDa, due to post translational modifications.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis using Rabbit Anti-Wnt1 Polyclonal Antibody. Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Wnt1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.