MA5-15544

antibody from Invitrogen Antibodies

Targeting: WNT1 INT1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15544

Provider

Invitrogen Antibodies

Product name

WNT1 Monoclonal Antibody (10C8)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15544 targets WNT1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human and mouse samples. The MA5-15544 immunogen is purified recombinant fragment of WNT1 expressed in E. Coli. MA5-15544 detects WNT1 which has a predicted molecular weight of approximately 41kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

10C8

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

E74-like factor 3 suppresses microRNA-485-5p transcription to trigger growth and metastasis of ovarian cancer cells with the involvement of CLDN4/Wnt/β-catenin axis.

Kuang L, Li L
Saudi journal of biological sciences 2021 Aug;28(8):4137-4146

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Relaxin reverses maladaptive remodeling of the aged heart through Wnt-signaling.

Martin B, Gabris B, Barakat AF, Henry BL, Giannini M, Reddy RP, Wang X, Romero G, Salama G
Scientific reports 2019 Dec 6;9(1):18545

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WNT1 using WNT1 monoclonal antibody (Product # MA5-15544) in NIH/3T3 (1), 3T3L1 (2) and HeLa (3) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WNT1 using WNT1 monoclonal antibody (Product # MA5-15544) in NIH/3T3 (1), 3T3L1 (2) and HeLa (3) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WNT1 using WNT1 monoclonal antibody (Product # MA5-15544) in NIH/3T3 (1), 3T3L1 (2) and HeLa (3) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa (left) and 3T3-L1 (right) cells using WNT1 monoclonal antibody (Product # MA5-15544) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human adrenal tissues using WNT1 monoclonal antibody (Product # MA5-15544).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using WNT1 monoclonal antibody (Product # MA5-15544) (green) and negative control (purple).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 miR-485-5p targets CLDN4 to regulate the Wnt/beta-catenin signaling. A, binding site between miR-485-5p and CLDN4 predicted on Starbase ( http://starbase.sysu.edu.cn/ ); B, miR-485-5p expression profile in OC predicted on GEPIA ( http://gepia.cancer-pku.cn/ ); C, protein expression of CLDN4 in tumor tissues and the paired normal tissues determined by immunohistochemical staining (paired t test,** p < 0.01); D, CLDN4 expression in OC cells and SV40 cells measured by RT-qPCR; E-F, binding relationship between miR-485-5p and CLDN4 validated through a dual luciferase reporter gene assay (E) and an RIP assay (F) (two-way ANOVA,* p < 0.05); G, CLDN4 expression in OC cells after miR-485-5p mimic and LV-CLDN4 transfection determined by RT-qPCR (one-way ANOVA, * p < 0.05 compared to NC mimic, # p < 0.05 compared to miR-485-5p mimic + LV-NC); H, protein levels of Wnt1 and beta-catenin in OC cell lines after miR-485-5p mimic and LV-CLDN4 transfection evaluated using western blot analysis (two-way ANOVA,* p < 0.05 compared to NC mimic, # p < 0.05 compared to miR-485-5p mimic + LV-NC). Data were exhibited as mean +- SD from three independent experiments.