Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [3]
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- Product number
- MA5-17149 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIK3CA Monoclonal Antibody (4F3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4F3
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Role of Periostin Expression in Non-Small Cell Lung Cancer: Periostin Silencing Inhibits the Migration and Invasion of Lung Cancer Cells via Regulation of MMP-2 Expression.
Silencing of soluble epoxide hydrolase 2 gene reduces H(2)O(2)-induced oxidative damage in rat intestinal epithelial IEC-6 cells via activating PI3K/Akt/GSK3β signaling pathway.
The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway.
Silence of α1-Antitrypsin Inhibits Migration and Proliferation of Triple Negative Breast Cancer Cells.
LncRNA DANCR upregulates PI3K/AKT signaling through activating serine phosphorylation of RXRA.
Ratajczak-Wielgomas K, Kmiecik A, Dziegiel P
International journal of molecular sciences 2022 Jan 22;23(3)
International journal of molecular sciences 2022 Jan 22;23(3)
Silencing of soluble epoxide hydrolase 2 gene reduces H(2)O(2)-induced oxidative damage in rat intestinal epithelial IEC-6 cells via activating PI3K/Akt/GSK3β signaling pathway.
Li J, Luo J, Zhang Y, Tang C, Wang J, Chen C
Cytotechnology 2020 Feb;72(1):23-36
Cytotechnology 2020 Feb;72(1):23-36
The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway.
Shen P, Chen J, Pan M
Molecular medicine reports 2018 Sep;18(3):3332-3340
Molecular medicine reports 2018 Sep;18(3):3332-3340
Silence of α1-Antitrypsin Inhibits Migration and Proliferation of Triple Negative Breast Cancer Cells.
Zhao Z, Ma J, Mao Y, Dong L, Li S, Zhang Y
Medical science monitor : international medical journal of experimental and clinical research 2018 Sep 27;24:6851-6860
Medical science monitor : international medical journal of experimental and clinical research 2018 Sep 27;24:6851-6860
LncRNA DANCR upregulates PI3K/AKT signaling through activating serine phosphorylation of RXRA.
Tang J, Zhong G, Zhang H, Yu B, Wei F, Luo L, Kang Y, Wu J, Jiang J, Li Y, Wu S, Jia Y, Liang X, Bi A
Cell death & disease 2018 Dec 5;9(12):1167
Cell death & disease 2018 Dec 5;9(12):1167
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3CA using a PIK3CA monoclonal antibody (Product # MA5-17149) against a human PIK3CA (AA: 881-1068) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3CA using PIK3CA monoclonal antibody (Product # MA5-17149) in HEK293 (1) and PIK3CA (AA: 881-1068) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3CA using PIK3CA monoclonal antibody (Product # MA5-17149) in HEK293 (1) and PIK3CA (AA: 881-1068) human IgG Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using PIK3CA monoclonal antibody (Product # MA5-17149) (Green). Blue: DRAQ5 fluorescent DNA dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using PIK3CA monoclonal antibody (Product # MA5-17149) (green) and negative control (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. TPG suppresses the PI3K/Akt signaling pathway in I/R-induced H9C2 cells. H9C2 cells were treated with TPG (10, 40 and 160 ug/ml) for 24 h and subjected to I/R injury. (A) Relative protein expression levels of p-PI3K, PI3K, p-Akt and Akt were assessed by western blotting. beta-actin was used as an internal control. (B) Blot results were semi-quantified. *P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 si-alpha1-AT blocked the PI3K/Akt/mTOR pathway. ( A ) The protein levels of PI3K and p-PI3K were determined using western bolt. ( B ) The protein levels of p-Akt and Akt were assessed using western bolt. ( C ) The protein levels of p-mTOR, and mTOR were measured using western bolt. * P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 11 Effects of periostin (POSTN) silencing on integrin-signaling-pathway-related protein. Western blotting assay was performed to detect integrin-alphavbeta3, p-AKT, total AKT, and PI3K in A549.shRNA cells compared to A549.CTRL cells. beta-actin protein was used as loading controls.