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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- LS-C99835 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C99835, RRID:AB_2165271
- Product name
- PIK3CA / PI3K Alpha Antibody (aa504-533) LS-C99835
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of anti-PI3KCA Antibody antibody in 293 cell line lysates transiently transfected with the PIK3CA gene (1 ug/lane). PI3KCA (arrow) was detected using the purified antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of anti-PIK3CA Antibody antibody in K562 cell line lysates (35 ug/lane). PIK3CA (arrow) was detected using the purified Pab
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
