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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP8016c - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP8016c, RRID:AB_2163890
- Product name
- PI3KCA Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 1.57 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-PIK3CA Antibody (Center) Pab (Cat.#AP8016c) in K562 cell line lysates (35ug/lane). PIK3CA (arrow) was detected using the purified Pab
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-PI3KCA Antibody (Center) Pab (Cat.#AP8016c) in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane). PI3KCA (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody (Center)(Cat.#AP8016c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100
