AP32486PU-N

antibody from Acris Antibodies GmbH

Targeting: PIK3CA PI3K

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

AP32486PU-N

Provider

Acris Antibodies GmbH

Product name

anti PIK3CA

Antibody type

Polyclonal

Antigen

KLH-conjugated, synthetic peptide corresponding to amino acids 1052-1068 of human 110kDa PI3 Kinase, with an N-terminal cysteine added for conjugation purposes.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.2 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunoprecipitation/Immunoblot Analysis: PI3 Kinase was immunoprecipitated from 500μg of EGF-stimulated A431 cell lysate with 5μg of anti-PI3 Kinase, p110α, resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PI3 Kinase, p85, N-SH3,clone AB6 (1μg/ml) proteins were visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and achemiluminescence detection system. Arrow indicates PI3 regulatory subunit (85kDa).