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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C356254 - Provider product page
- Provider
- LSBio
- Product name
- PIK3CA / PI3K Alpha Antibody (Internal) LS-C356254
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Immunohistochemical analysis of PI3K p110 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
