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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB2126 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2126, RRID:AB_1578760
- Product name
- PIK3CA polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PIK3CA.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Activated G alpha q inhibits p110 alpha phosphatidylinositol 3-kinase and Akt.
p53 regulates cell survival by inhibiting PIK3CA in squamous cell carcinomas.
PIK3CA is implicated as an oncogene in ovarian cancer.
Ballou LM, Lin HY, Fan G, Jiang YP, Lin RZ
The Journal of biological chemistry 2003 Jun 27;278(26):23472-9
The Journal of biological chemistry 2003 Jun 27;278(26):23472-9
p53 regulates cell survival by inhibiting PIK3CA in squamous cell carcinomas.
Singh B, Reddy PG, Goberdhan A, Walsh C, Dao S, Ngai I, Chou TC, O-Charoenrat P, Levine AJ, Rao PH, Stoffel A
Genes & development 2002 Apr 15;16(8):984-93
Genes & development 2002 Apr 15;16(8):984-93
PIK3CA is implicated as an oncogene in ovarian cancer.
Shayesteh L, Lu Y, Kuo WL, Baldocchi R, Godfrey T, Collins C, Pinkel D, Powell B, Mills GB, Gray JW
Nature genetics 1999 Jan;21(1):99-102
Nature genetics 1999 Jan;21(1):99-102
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3CA polyclonal antibody (Cat # PAB2126) in K-562 cell line lysates (35 ug/lane). PIK3CA (arrow) was detected using the purified Polyclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3CA (arrow) using PIK3CA polyclonal antibody (Cat # PAB2126) in 293 cell line lysates transiently transfected with the PIK3CA gene (1 ug/lane). PIK3CA (arrow) was detected using the purified Polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PIK3CA polyclonal antibody (Cat # PAB2126) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
