Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27192 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PIK3CA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-27192 targets PI3 kinase p110 alpha in IHC (P) and WB applications and shows reactivity with Human and mouse samples. The PA5-27192 immunogen is recombinant protein fragment corresponding to a region within amino acids 3 and 170 of Human PI3 kinase p110 alpha.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.98 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Snail heterogeneity in clear cell renal cell carcinoma.
Zaldumbide L, Erramuzpe A, Guarch R, Pulido R, Cortés JM, López JI
BMC cancer 2016 Mar 8;16:194
BMC cancer 2016 Mar 8;16:194
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PI3 kinase p110 alpha using 50µg of A) C2C12 and B) C2C12+AEA10mM lysate. Samples were probed with a PI3 kinase p110 alpha polyclonal antibody (Product # PA5-27192) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PI3 kinase p110 alpha using 30µg of HCT116 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PI3 kinase p110 alpha polyclonal antibody (Product # PA5-27192) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PI3 kinase p110 alpha in paraffin-embedded human breast carcinoma using a PI3 kinase p110 alpha polyclonal antibody (Product # PA5-27192).