Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-21522 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSD17B4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Rat heart. Predicted reactivity: Mouse (87%), Rat (88%), Pig (88%), Bovine (88%), Guinea pig (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Regulatory Functions of L-Carnitine, Acetyl, and Propionyl L-Carnitine in a PCOS Mouse Model: Focus on Antioxidant/Antiglycative Molecular Pathways in the Ovarian Microenvironment.
Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice.
Di Emidio G, Rea F, Placidi M, Rossi G, Cocciolone D, Virmani A, Macchiarelli G, Palmerini MG, D'Alessandro AM, Artini PG, Tatone C
Antioxidants (Basel, Switzerland) 2020 Sep 15;9(9)
Antioxidants (Basel, Switzerland) 2020 Sep 15;9(9)
Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice.
Emidio GD, Placidi M, Rea F, Rossi G, Falone S, Cristiano L, Nottola S, D'Alessandro AM, Amicarelli F, Palmerini MG, Tatone C
Cells 2020 Jan 14;9(1)
Cells 2020 Jan 14;9(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HSD17B4 Polyclonal Antibody detects HSD17B4 protein by western blot analysis. A. 20 µg Rat heart lysate/extract. 7.5% SDS-PAGE. HSD17B4 Polyclonal Antibody (Product # PA5-21522) dilution: 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 17beta-HSD4 in methanol-fixed Hep3B cells using a 17beta-HSD4 polyclonal antibody (Product # PA5-21522) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed Hep3B, using HSD17B4 antibody (Product # PA5-21522) at 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of HSD17B4 was performed in paraffin-embedded rat testis tissue using HSD17B4 Polyclonal Antibody (Product # PA5-21522) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of HSD17B4 was performed in paraffin-embedded rat heart tissue using HSD17B4 Polyclonal Antibody (Product # PA5-21522) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using HSD17B4 (Product # PA5-21522) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.