PA5-48055

antibody from Invitrogen Antibodies

Targeting: TGM3 TGE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-48055

Provider

Invitrogen Antibodies

Product name

TGM3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) TGM2, rhTGM4, and rhTGM7 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of B16-F1 mouse melanoma cell line, Bowes human melanoma cell line, and human skin tissue. PVDF Membrane was probed with 1 µg/mL of human Transglutaminase 3/TGM3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48055) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. For additional reference, recombinant human Transglutaminase 3/TGM3 (10 ng/lane) uncleaved and cleaved by recombinant human Cathepsin were included. Specific bands were detected for uncleaved Transglutaminase 3/TGM3 at approximately 77 kDa (as indicated) and Transglutaminase 3/TGM3 cleavage products at approximately 50 and 30 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TGM3 in B16‚F1 mouse melanoma cell line, Bowes human melanoma cell line, and human skin tissue. Samples were incubated in TGM3 polyclonal antibody (Product # PA5-48055) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. For additional reference, Recombinant Human Transglutaminase 3/TGM3 (10 ng/lane) uncleaved and cleaved by recombinant human Cathepsin were included. Specific bands were detected for uncleaved Transglutaminase 3/TGM3 at approximately 77 kDa (as indicated) and Transglutaminase 3/TGM3 cleavage products at approximately 50 and 30 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of TGM3 in immersion fixed paraffin-embedded sections of human esophagus. Samples were incubated in TGM3 polyclonal antibody (Product # PA5-48055) using a dilution of 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cells in the papillae layer in mucosa.