GTX112232
antibody from GeneTex
Targeting: ZHX2
KIAA0854
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- GTX112232 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112232, RRID:AB_1952653
- Product name
- ZHX2 antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 7.5% SDS PAGE GTX112232 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZHX2(GTX112232) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZHX2 antibody [C1C3] detects ZHX2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ZHX2 protein stained by ZHX2 antibody [C1C3] (GTX112232) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ZHX2 protein from MCF-7 whole cell extracts using 5 £gg of ZHX2 antibody [C1C3] (GTX112232).Western blot analysis was performed using ZHX2 antibody [C1C3] (GTX112232).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using ZHX2(GTX112232) antibody at 1:100 dilution.