MA5-23522

antibody from Invitrogen Antibodies

Targeting: BICD2 KIAA0699

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23522

Provider

Invitrogen Antibodies

Product name

BICD2 Monoclonal Antibody (3I3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, HeLa, and HepG2 cells. Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3I3

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Cytoplasmic control of intranuclear polarity by human cytomegalovirus.

Procter DJ, Furey C, Garza-Gongora AG, Kosak ST, Walsh D
Nature 2020 Nov;587(7832):109-114

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MiR-4674 regulates angiogenesis in tissue injury by targeting p38K signaling in endothelial cells.

Icli B, Li H, Pérez-Cremades D, Wu W, Ozdemir D, Haemmig S, Guimaraes RB, Manica A, Marchini JF, Orgill DP, Feinberg MW
American journal of physiology. Cell physiology 2020 Mar 1;318(3):C524-C535

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of BICD2 was performed in HeLa cells fixed in 4% paraformaldehyde for 10 min. Green: BICD2 Monoclonal Antibody (3I3) (Product # MA5-23522) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of BICD2 was performed in HeLa cells fixed in 4% paraformaldehyde for 10 min. Green: BICD2 Monoclonal Antibody (3I3) (Product # MA5-23522) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Extended Data Fig. 3: SUN1:Nesprin-2G and the dynein adaptor BICD2 mediate nuclear rotation during HCMV infection. a-b, Expression of a SUN1 mutant that does not engage Nesprin-2G impairs nuclear rotation in HCMV-infected cells. a, Representative stills from time lapse recordings of NHDFs expressing Tag-GFP2 forms of SUN1 Full Length (FL) or SUN1 lacking the lumenal domain (SUN1DeltaLu) that mediates interactions with Nesprin-2G, infected with HCMV-UL99mCherry. Rotation traces from this imaging are shown to the right. Analyses focused on cells expressing intermediate levels of SUN1-GFP constructs as high levels of expression can result in retention of Nesprin-2G in the endoplasmic reticulum (ER). b, Quantification of rotation frequencies above or below 180deg; bars represent mean +- SEM, statistics use two-tailed student's t-test, n = 138 cells total from 3 independent biological replicates, ***pLEAA kinesin-binding mutant. e, Western blot analysis of BICD2 expressio