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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 18142-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HRC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTQQLRGDG LGFRNRNNST GVAGLSEEAS AELRHHLHSP RDHPDENKDV STENGHHFWS HPDREKEDED VSKEYGHLLP GHRSQDHKVG DEGVSGEEVF AEHGGQARGH RGHGSEDTED SAEHRHHLPS HRSHSHQDED EDEVVSSEHH HHILRHGHRG HDGEDDEGEE EEEEEEEEEE ASTEYGHQAH RHRGHGSEED EDVSDGHHHH GPSHRHQGHE EDDDDDDDDD DDDDDDDVSI EYRHQAHRHQ GHGIEEDEDV SDGHHHRDPS HRHRSHEEDD NDDDDVSTEY GHQAHRHQDH RKEEVEAVSG EHHHHVPDHR HQGHRD (26-350 aa encoded by BC069795)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.35 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 18142-1-AP (HRC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
