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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA1-29829 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMAD6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-29829 detects SMAD6 from mouse, human samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD6 in Jurkat cell lysate using a SMAD6 polyclonal antibody (Product # PA1-29829) at a dilution of 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SMAD6 was performed by loading HepG2 cell lysate. Proteins were transferred to a membrane and probed with a SMAD6 Polyclonal Antibody (Product # PA1-29829).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SMAD6 was performed by loading Jurkat cell lysates. Proteins were transferred to a membrane and probed with a SMAD6 Polyclonal Antibody (Product # PA1-29829) at a dilution of 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SMAD6 in HepG2 cells. Cells were fixed with 10% formalin, permeabilized with 0.5% Triton-X100 and SMAD6 Polyclonal Antibody (Product # PA1-29829) (Green) at a dilution of 1:100 was used as a primary antibody. Red : Tubulin. Blue: DAPI.
