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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA1-41026 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMAD6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with equine based on sequence homology. Suggested positive control: HeLa or NIH-3T3 whole cell lysate.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The transcriptomic and epigenetic map of vascular quiescence in the continuous lung endothelium.
Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats.
Schlereth K, Weichenhan D, Bauer T, Heumann T, Giannakouri E, Lipka D, Jaeger S, Schlesner M, Aloy P, Eils R, Plass C, Augustin HG
eLife 2018 May 11;7
eLife 2018 May 11;7
Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats.
Beaudoin MS, Snook LA, Arkell AM, Stefanson A, Wan Z, Simpson JA, Holloway GP, Wright DC
Obesity (Silver Spring, Md.) 2014 Jul;22(7):1632-42
Obesity (Silver Spring, Md.) 2014 Jul;22(7):1632-42
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis ofSMAD6 on Lysate fromJurkat cells using a SMAD6 polyclonal antibody (Product # PA1-41026) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD6 in Lysate from Jurkat cells. Samples were incubated in SMAD6 polyclonal antibody (Product # PA1-41026) using a dilution of 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD6 in 0.5 mg/mL HepG2 lysate. Samples were incubated in SMAD6 polyclonal (Product # PA1-41026). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SMAD6 in immersion fixed paraffin-embedded sections of human lung. Samples were incubated in SMAD6 polyclonal antibody (Product # PA1-41026) using a dilution of 5 µg/mL for 1 hour at room temperature followed by Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Nuclear staining was localized to the pneumocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Author response image 2. HUVEC were either depleted for ( A, B ) or overexpressing ( C, D ) SMAD6 and/or SMAD7. Both, shRNAs or overexpression constructs were introduced by lentiviral transduction followed by selection with puromycin (P) or neomycin (N). Panel A and C represents Taqman-based qPCR data and panel B and D the corresponding protein expression data from the same treatment replicates. SMAD6 antibody from Life Technologies (PA1-41026), SMAD7 antibody from R&D systems (MAB2029).
