Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF4894 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EMR2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human EMR2 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows less than 1% cross-reactivity with recombinant mouse EMR1.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_038475
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human EMR2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMR2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by EMR2 and Neutralization by Human EMR2 Antibody. Recombinant Human EMR2 (Catalog # 4894-EM), immobilized onto a microplate, supports the adhesion of the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human EMR2 (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894). The ND50 is typically 1.5-6 µg/mL.