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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX115295 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115295, RRID:AB_11176555
- Product name
- RNF40 antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE GTX115295 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RNF40 antibody detects RNF40 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RNF40 antibody (GTX115295) diluted by 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using RNF40(GTX115295) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of RNF40 protein from HeLa whole cell extracts using 5 £gg of RNF40 antibody [N1N2], N-term (GTX115295).Western blot analysis was performed using RNF40 antibody [N1N2], N-term (GTX115295).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RNF40(GTX115295) antibody at 1:250 dilution.
