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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- 701142 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDGFRA Recombinant Rabbit Monoclonal Antibody (7H13L1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
- Antibody clone number
- 7H13L1
- Concentration
- 0.5 mg/mL
Submitted references Rhythmic Calcium Events in the Lamina Propria Network of the Urinary Bladder of Rat Pups.
EGF as a New Therapeutic Target for Medulloblastoma Metastasis.
Heppner TJ, Hennig GW, Nelson MT, Vizzard MA
Frontiers in systems neuroscience 2017;11:87
Frontiers in systems neuroscience 2017;11:87
EGF as a New Therapeutic Target for Medulloblastoma Metastasis.
Rico-Varela J, Singh T, McCutcheon S, Vazquez M
Cellular and molecular bioengineering 2015 Dec;8(4):553-565
Cellular and molecular bioengineering 2015 Dec;8(4):553-565
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDGFR alpha in whole cell extracts of HepG2 using a PDGFR alpha recombinant rabbit monoclonal antibody (Product # 701142) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~190kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PDGFR alpha in HeLa cells using a PDGFR alpha recombinant rabbit monoclonal antibody (Product # 701142) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic and nuclear membrane localization of PDGFR-alpha.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of PDGF Receptor alpha was performed using cross-linked chromatin from 1 x 10^6 HCT116 human colon carcinoma cells treated with serum for 0, 15, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of a PDGF Receptor alpha rabbit monoclonal antibody (Product # 701142). Chromatin aliquots from ~1 x 10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -3.2kb upstream of the human FOS gene, or exon-4 of human FOS. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the FOS locus is shown above the data where boxes represent exons (grey boxes = translated regions, white boxes = untranslated regions), the zigzag lines represent introns, and the straight line represents upstream sequence. Regions amplified by FOS primers are represented by black bars. Data courtesy of the Innovators Program.
