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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
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- Product number
- PB9771 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-PDGF Receptor alpha/PDGFRA Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for PDGF RECEPTOR ALPHA/PDGFRA detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. PDGF RECEPTOR ALPHA/PDGFRA information: Molecular Weight: 122670 MW; Subcellular Localization: Cell membrane ; Single- pass type I membrane protein . The activated receptor is rapidly internalized and degraded; Tissue Specificity: Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Janus Kinase 1 Plays a Critical Role in Mammary Cancer Progression.
Loss of ATRX suppresses ATM dependent DNA damage repair by modulating H3K9me3 to enhance temozolomide sensitivity in glioma.
PDGFRα Regulated by miR-34a and FoxO1 Promotes Adipogenesis in Porcine Intramuscular Preadipocytes through Erk Signaling Pathway.
Wehde BL, Rädler PD, Shrestha H, Johnson SJ, Triplett AA, Wagner KU
Cell reports 2018 Nov 20;25(8):2192-2207.e5
Cell reports 2018 Nov 20;25(8):2192-2207.e5
Loss of ATRX suppresses ATM dependent DNA damage repair by modulating H3K9me3 to enhance temozolomide sensitivity in glioma.
Han B, Cai J, Gao W, Meng X, Gao F, Wu P, Duan C, Wang R, Dinislam M, Lin L, Kang C, Jiang C
Cancer letters 2018 Apr 10;419:280-290
Cancer letters 2018 Apr 10;419:280-290
PDGFRα Regulated by miR-34a and FoxO1 Promotes Adipogenesis in Porcine Intramuscular Preadipocytes through Erk Signaling Pathway.
Sun YM, Qin J, Liu SG, Cai R, Chen XC, Wang XM, Pang WJ
International journal of molecular sciences 2017 Nov 15;18(11)
International journal of molecular sciences 2017 Nov 15;18(11)
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of PDGFRA using anti-PDGFRA antibody (PB9771). PDGFRA was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDGFRA Antibody (PB9771) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
