Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- A304-729A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCT7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CCT7 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- CCT7 (Cat. No. A304-729A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CCT7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCT7 antibody A304-729A (lot A304-729A-1) used for IP at 6 µg per reaction. CCT7 was also immunoprecipitated by rabbit anti-CCT7 antibody A304-730A. For blotting immunoprecipitated CCT7, A304-729A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.