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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-27393 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TCP-1 eta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, Jurkat. Predicted reactivity: Mouse (96%), Rat (97%), Zebrafish (92%), Xenopus laevis (93%), Pig (100%), Rabbit (98%), Chicken (94%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TCP1 eta using A) 30 µg 293T whole cell extract (B) 30 µg A431 whole cell extract (C) 30 µg HeLa whole cell extract and D) 30 µg HepG2 whole cell extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TCP1 eta polyclonal antibody (Product # PA5-27393) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TCP-1 eta Polyclonal Antibody (Product # PA5-27393). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP-1 eta Polyclonal Antibody (Product # PA5-27393) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
