Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- HPA008425 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA008425, RRID:AB_1078428
- Product name
- Anti-CCT7
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human CCT7, Gene description: chaperonin containing TCP1, subunit 7 (eta), Alternative Gene Names: Ccth, Nip7-1, Validated applications: ICC, WB, IHC, Uniprot ID: Q99832, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Polycomb Requires Chaperonin Containing TCP-1 Subunit 7 for Maintaining Gene Silencing in Drosophila.
CCT complex restricts neuropathogenic protein aggregation via autophagy
Germline Variants in Asporin Vary by Race, Modulate the Tumor Microenvironment, and Are Differentially Associated with Metastatic Prostate Cancer
Dysfunctional nitric oxide signalling increases risk of myocardial infarction
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Shaheen N, Akhtar J, Umer Z, Khan MHF, Bakhtiari MH, Saleem M, Faisal A, Tariq M
Frontiers in cell and developmental biology 2021;9:727972
Frontiers in cell and developmental biology 2021;9:727972
CCT complex restricts neuropathogenic protein aggregation via autophagy
Pavel M, Imarisio S, Menzies F, Jimenez-Sanchez M, Siddiqi F, Wu X, Renna M, O’Kane C, Crowther D, Rubinsztein D
Nature Communications 2016;7(1)
Nature Communications 2016;7(1)
Germline Variants in Asporin Vary by Race, Modulate the Tumor Microenvironment, and Are Differentially Associated with Metastatic Prostate Cancer
Hurley P, Sundi D, Shinder B, Simons B, Hughes R, Miller R, Benzon B, Faraj S, Netto G, Vergara I, Erho N, Davicioni E, Karnes R, Yan G, Ewing C, Isaacs S, Berman D, Rider J, Jordahl K, Mucci L, Huang J, An S, Park B, Isaacs W, Marchionni L, Ross A, Schaeffer E
Clinical Cancer Research 2016;22(2):448-458
Clinical Cancer Research 2016;22(2):448-458
Dysfunctional nitric oxide signalling increases risk of myocardial infarction
Erdmann J, Stark K, Esslinger U, Rumpf P, Koesling D, de Wit C, Kaiser F, Braunholz D, Medack A, Fischer M, Zimmermann M, Tennstedt S, Graf E, Eck S, Aherrahrou Z, Nahrstaedt J, Willenborg C, Bruse P, Brænne I, Nöthen M, Hofmann P, Braund P, Mergia E, Reinhard W, Burgdorf C, Schreiber S, Balmforth A, Hall A, Bertram L, Steinhagen-Thiessen E, Li S, März W, Reilly M, Kathiresan S, McPherson R, Walter U, Ott J, Samani N, Strom T, Meitinger T, Hengstenberg C, Schunkert H
Nature 2013;504(7480):432-436
Nature 2013;504(7480):432-436
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251
Journal of Proteomics 2012;75(7):2236-2251
No comments: Submit comment
Enhanced validation
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting CCT7 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting CCT7 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:58
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CCT7 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
