NBP2-14786
antibody from Novus Biologicals
Targeting: ATAD2
ANCCA, CT137, DKFZp667N1320, MGC29843, MGC5254, PRO2000
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP2-14786 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ATAD2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human ATAD2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
VGDKRSDPEQ
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786] - Simple Western lane view shows a specific band for ATAD2 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786] - Electropherogram image(s) of corresponding Simple Western lane view. ATAD2 antibody was used at 1:20 dilution on U-251MG sp lysates(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATAD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human rectum shows strong nuclear positivity in a subset of glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining in human testis and skeletal muscle tissues. Corresponding ATAD2 RNA-seq data are presented for the same tissues.