NB600-636

Stanzani A, Galiazzo G, Giancola F, Tagliavia C, De Silva M, Pietra M, Fracassi F, Chiocchetti R
Histochemistry and cell biology 2020 May;153(5):339-356

Bromocriptine mesylate improves glucose tolerance and disposal in a high-fat-fed canine model.

Moore MC, Smith MS, Swift LL, Cincotta AH, Ezrokhi M, Cominos N, Zhang Y, Farmer B, Cherrington AD
American journal of physiology. Endocrinology and metabolism 2020 Jul 1;319(1):E133-E145

Cellular Distribution of Canonical and Putative Cannabinoid Receptors in Canine Cervical Dorsal Root Ganglia.

Chiocchetti R, Galiazzo G, Tagliavia C, Stanzani A, Giancola F, Menchetti M, Militerno G, Bernardini C, Forni M, Mandrioli L
Frontiers in veterinary science 2019;6:313

CBX5/G9a/H3K9me-mediated gene repression is essential to fibroblast activation during lung fibrosis.

Ligresti G, Caporarello N, Meridew JA, Jones DL, Tan Q, Choi KM, Haak AJ, Aravamudhan A, Roden AC, Prakash YS, Lomberk G, Urrutia RA, Tschumperlin DJ
JCI insight 2019 May 16;5

Troglitazone activates TRPV1 and causes deacetylation of PPARγ in 3T3-L1 cells.

Krishnan V, Baskaran P, Thyagarajan B
Biochimica et biophysica acta. Molecular basis of disease 2019 Feb 1;1865(2):445-453

Binding Efficacy and Thermogenic Efficiency of Pungent and Nonpungent Analogs of Capsaicin.

Baskaran P, Covington K, Bennis J, Mohandass A, Lehmann T, Thyagarajan B
Molecules (Basel, Switzerland) 2018 Dec 4;23(12)

Localization of cannabinoid receptors CB1, CB2, GPR55, and PPARα in the canine gastrointestinal tract.

Galiazzo G, Giancola F, Stanzani A, Fracassi F, Bernardini C, Forni M, Pietra M, Chiocchetti R
Histochemistry and cell biology 2018 Aug;150(2):187-205

Pharmacological inhibition of NOX4 ameliorates alcohol-induced liver injury in mice through improving oxidative stress and mitochondrial function.

Sun Q, Zhang W, Zhong W, Sun X, Zhou Z
Biochimica et biophysica acta. General subjects 2017 Jan;1861(1 Pt A):2912-2921

Editor's Highlight: Neonatal Activation of the Xenobiotic-Sensors PXR and CAR Results in Acute and Persistent Down-regulation of PPARα-Signaling in Mouse Liver.

Li CY, Cheng SL, Bammler TK, Cui JY
Toxicological sciences : an official journal of the Society of Toxicology 2016 Oct;153(2):282-302

Hyperactive mTORC1 signaling is unaffected by metformin treatment in aged skeletal muscle.

Dungan CM, Li Z, Wright DC, Williamson DL
Muscle & nerve 2016 Jan;53(1):107-17

Regulation of Hepatic Drug-Metabolizing Enzymes in Germ-Free Mice by Conventionalization and Probiotics.

Selwyn FP, Cheng SL, Klaassen CD, Cui JY
Drug metabolism and disposition: the biological fate of chemicals 2016 Feb;44(2):262-74

Capsaicin induces browning of white adipose tissue and counters obesity by activating TRPV1 channel-dependent mechanisms.

Baskaran P, Krishnan V, Ren J, Thyagarajan B
British journal of pharmacology 2016 Aug;173(15):2369-89

Alpha-lipoic acid supplementation reduces mTORC1 signaling in skeletal muscle from high fat fed, obese Zucker rats.

Li Z, Dungan CM, Carrier B, Rideout TC, Williamson DL
Lipids 2014 Dec;49(12):1193-201

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PPAR alpha/NR1C1 Antibody [NB600-636] - Affinity Purified Detection a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Storage Conditions: Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PPAR alpha/NR1C1 Antibody [NB600-636] - Lane 1: NIH/3T3. Load: 10 ug per lane. Primary antibody: PPAR Alpha (N-terminal specific) antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting at RT for 30 min. Predicted/Observed size: 50 kDa for PPAR Alpha.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: ELISA: PPAR alpha/NR1C1 Antibody [NB600-636] - ELISA results of purified Rabbit anti-PPAR Alpha (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NB600-636] - Rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventricle (exit point of progenitor olfactory neurons); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NB600-636] - Mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).