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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA503529 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503529, RRID:AB_11128618
- Product name
- PPARA (PPAR alpha) mouse monoclonal antibody, clone OTI2D10 (formerly 2D10)
- Antibody type
- Monoclonal
- Description
- PPARA (PPAR alpha) mouse monoclonal antibody, clone OTI2D10 (formerly 2D10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PPARA
- Isotype
- IgG
- Antibody clone number
- OTI2D10
- Vial size
- 100 µl
- Concentration
- 0.48 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (RC216176, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PPARA mouse monoclonal antibody (TA503529) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA(RC216176).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC216176 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPARA antibody(TA503529), and then analyzed by flow cytometry.
- Validation comment
- FC
