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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ4086 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GALT Antibody / Galactose-1-phosphate uridyltransferase
- Antibody type
- Polyclonal
- Description
- This highly specific GALT antibody is suitable for use in Western blot/Immunohistochemistry/Immunoprecipitation/Flow cytometry/ELISA applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the GALT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HepG2 and 2) MCF7 cell lysate with GALT antibody at 0.5ug/ml. Predicted molecular weight ~43 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human ThP-1, 2) human HepG2, 3) human HeLa, 4) rat lung abd 5) rat kidney tissue lysate with GALT antibody. Predicted molecular weight ~43 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation of GALT protein from 500ug of human ThP-1 whole cell lysate with 2ug of GALT antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colon cancer tissue with GALT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human HepG2 cells with GALT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GALT antibody.
