NBP1-50001

antibody from Novus Biologicals

Targeting: USP8 HumORF8, KIAA0055, SPG59, UBPY

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-50001

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-50001, RRID:AB_10012191

Product name

Rabbit Polyclonal UBPY/USP8 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: UBPY/USP8 Antibody [NBP1-50001] - Detection of Human USP8 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-USP8 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. USP8 was also immunoprecipitated by rabbit anti-USP8 antibody which recognizes a downstream epitope. For blotting immunoprecipitated USP8, the ReliaBLOT(R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBPY/USP8 Antibody [NBP1-50001] - Detection of human USP8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-USP8 (NBP1-50001) used at a dilution of 1:200 (1ug/ml). Detection: DAB