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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27947 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, HCT116. Predicted reactivity: Rhesus Monkey (95%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references TMEM79/MATTRIN defines a pathway for Frizzled regulation and is required for Xenopus embryogenesis.
Chen M, Amado N, Tan J, Reis A, Ge M, Abreu JG, He X
eLife 2020 Sep 14;9
eLife 2020 Sep 14;9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP8 using 30 µg of A) H1299 and B) HCT116 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a USP8 polyclonal antibody (Product # PA5-27947) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of USP8 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a USP8 Polyclonal Antibody (Product # PA5-27947) at a dilution of 1:5000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- USP8 Polyclonal Antibody detects USP8 protein at cytoplasm by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP8 protein stained by USP8 Polyclonal Antibody (Product # PA5-27947) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using USP8 (Product # PA5-27947) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
