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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347083 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal BCL7C Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal BCL7C Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- BCL7C
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using the antibody against BCL7C diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The antibody detects both known isoforms of the protein (expected sizes: 23.4 and 26.4 kDa).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human BCL7C. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:209,000.
- Validation comment
- ELISA
