A300-289A

antibody from Invitrogen Antibodies

Targeting: SETD1A KIAA0339, KMT2F, Set1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-289A

Provider

Invitrogen Antibodies

Product name

hSET1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human hSET1 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-hSET1 antibody (Product # A300-289A; lot A300-289A-6) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human hSET1 by western blot of immunoprecipitates. Samples: Nuclear Extract (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-hSET1 antibody A300-289A (lot A300-289A-4) used for IP at 6 µg per reaction. hSET1 was also immunoprecipitated by rabbit anti-hSET1 antibody A300-288A. For blotting immunoprecipitated hSET1, A300-289A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human hSET1 by western blot. Samples: Nuclear extract (50, 15, 5 µg) from 293T cells. Antibody: Affinity purified rabbit anti-hSET1 antibody A300-289A (lot A300-289A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human hSET1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma.Antibody: Affinity purified rabbit anti-hSET1 (Product # A300-289A; lot A300-289A-6) used at 1:5,000 (0.2 µg/mL).Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human hSet1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-hSet1 Cat. No. A300-289A Lot4 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP-chip scatter plot of anti-hSET1 (A300-289A) enriched DNA binding sites versus input reference DNA. A. 10 µg of A300-289A was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-hSET1 ChIP, normal rabbit IgG showed little enrichment.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human hSET1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-hSET1 antibody (Product # A300-289A; lot A300-289A-6) used for IP at 6 µg per reaction. hSET1 was also immunoprecipitated by a previous lot of this antibody (lot A300-289A-5) and rabbit anti-hSET1 antibody (Product # A700-024). For blotting immunoprecipitated hSET1 (Product # A700-024) was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.