Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-29510 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSPH Recombinant Rabbit Monoclonal Antibody (1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Antibody clone number
- 1
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of PSPH in Lane A: MCF7 Whole Cell Lysate (30 µg), Lane B: K562 Whole Cell Lysate (30 µg), Lane C: HepG2 Whole Cell Lysate (30 µg). Samples were probed using a PSPH Monoclonal Antibody (Product # MA5-29510) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:25 kDa. Observed band size:27 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PSPH Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29510) at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate, Lane B: K562 Whole Cell Lysate, Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 27 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Human PSPH in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PSPH Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29510, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PSPH Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate 0.5 µL with PSPH Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29510) and 15 µL of 50 % Protein G agarose. Primary antibody: PSPH Recombinant Rabbit Monoclonal Antibody (1), at 1:1,000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa.