Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-29510 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSPH Recombinant Rabbit Monoclonal Antibody (001)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human PSPH.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 1
- Vial size
- 100 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSPH in Lane A: MCF7 Whole Cell Lysate (30 µg), Lane B: K562 Whole Cell Lysate (30 µg), Lane C: HepG2 Whole Cell Lysate (30 µg). Samples were probed using a PSPH Monoclonal Antibody (Product # MA5-29510) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:25 kDa. Observed band size:27 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PSPH Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29510) at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate, Lane B: K562 Whole Cell Lysate, Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 27 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Human PSPH in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PSPH Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29510, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PSPH Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate 0.5 µL with PSPH Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29510) and 15 µL of 50 % Protein G agarose. Primary antibody: PSPH Recombinant Rabbit Monoclonal Antibody (1), at 1:1,000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa.