Antibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 14631-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14631-1-AP, RRID:AB_2242420
- Product name
- AFG3L2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated AFG3L2 antibody (Cat. #14631-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition.
Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis.
LonP1 Links Mitochondria-ER Interaction to Regulate Heart Function.
FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes.
LPGAT1 controls MEGDEL syndrome by coupling phosphatidylglycerol remodeling with mitochondrial transport.
Ferroptosis inducers enhanced cuproptosis induced by copper ionophores in primary liver cancer.
Leber's hereditary optic neuropathy-associated ND6 14484T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy.
Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.
Complementary anti-cancer pathways triggered by inhibition of sideroflexin 4 in ovarian cancer.
Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.
The mitochondrial UPR regulator ATF5 promotes intestinal barrier function via control of the satiety response.
A novel mutation located in the intermembrane space domain of AFG3L2 causes dominant optic atrophy through decreasing the stability of the encoded protein.
Translation of MT-ATP6 pathogenic variants reveals distinct regulatory consequences from the co-translational quality control of mitochondrial protein synthesis.
A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.
Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNA(His) mutation.
Expanding the clinical and genetic heterogeneity of SPAX5.
Sphingosine kinase 1 overexpression induces MFN2 fragmentation and alters mitochondrial matrix Ca(2+) handling in HeLa cells.
Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.
Mitochondrial stress response triggered by defects in protein synthesis quality control.
Fidelity of translation initiation is required for coordinated respiratory complex assembly.
Sideroflexin 4 affects Fe-S cluster biogenesis, iron metabolism, mitochondrial respiration and heme biosynthetic enzymes.
Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.
Adult-onset obesity is triggered by impaired mitochondrial gene expression.
Proteolytic control of the mitochondrial calcium uniporter complex.
Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.
Novel MTND1 mutations cause isolated exercise intolerance, complex I deficiency and increased assembly factor expression.
Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.
Fang L, Zhang L, Wang M, He Y, Yang J, Huang Z, Tan Y, Fang K, Li J, Sun Z, Li Y, Tang Y, Liang W, Cui H, Zhu Q, Wu Z, Li Y, Hu Y, Chen W
Cancer research 2024 Mar 4;84(5):659-674
Cancer research 2024 Mar 4;84(5):659-674
Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis.
Shi X, DeCiucis M, Grabinska KA, Kanyo J, Liu A, Lam TT, Shen H
Molecular cell 2024 Feb 15;84(4):802-810.e6
Molecular cell 2024 Feb 15;84(4):802-810.e6
LonP1 Links Mitochondria-ER Interaction to Regulate Heart Function.
Li Y, Huang D, Jia L, Shangguan F, Gong S, Lan L, Song Z, Xu J, Yan C, Chen T, Tan Y, Liu Y, Huang X, Suzuki CK, Yang Z, Yang G, Lu B
Research (Washington, D.C.) 2023;6:0175
Research (Washington, D.C.) 2023;6:0175
FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes.
Qiu J, Yue F, Zhu P, Chen J, Xu F, Zhang L, Kim KH, Snyder MM, Luo N, Xu HW, Huang F, Tao WA, Kuang S
Nature communications 2023 Oct 10;14(1):6344
Nature communications 2023 Oct 10;14(1):6344
LPGAT1 controls MEGDEL syndrome by coupling phosphatidylglycerol remodeling with mitochondrial transport.
Sun H, Zhang J, Ye Q, Jiang T, Liu X, Zhang X, Zeng F, Li J, Zheng Y, Han X, Su C, Shi Y
Cell reports 2023 Nov 28;42(11):113214
Cell reports 2023 Nov 28;42(11):113214
Ferroptosis inducers enhanced cuproptosis induced by copper ionophores in primary liver cancer.
Wang W, Lu K, Jiang X, Wei Q, Zhu L, Wang X, Jin H, Feng L
Journal of experimental & clinical cancer research : CR 2023 Jun 6;42(1):142
Journal of experimental & clinical cancer research : CR 2023 Jun 6;42(1):142
Leber's hereditary optic neuropathy-associated ND6 14484T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy.
Liang M, Ji Y, Zhang L, Wang X, Hu C, Zhang J, Zhu Y, Mo JQ, Guan MX
Human molecular genetics 2022 Sep 29;31(19):3299-3312
Human molecular genetics 2022 Sep 29;31(19):3299-3312
Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.
Tsai CW, Rodriguez MX, Van Keuren AM, Phillips CB, Shushunov HM, Lee JE, Garcia AM, Ambardekar AV, Cleveland JC Jr, Reisz JA, Proenza C, Chatfield KC, Tsai MF
Molecular cell 2022 Oct 6;82(19):3661-3676.e8
Molecular cell 2022 Oct 6;82(19):3661-3676.e8
Complementary anti-cancer pathways triggered by inhibition of sideroflexin 4 in ovarian cancer.
Tesfay L, Paul BT, Hegde P, Brewer M, Habbani S, Jellison E, Moore T, Wu H, Torti SV, Torti FM
Scientific reports 2022 Nov 19;12(1):19936
Scientific reports 2022 Nov 19;12(1):19936
Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.
Liu H, Fan H, He P, Zhuang H, Liu X, Chen M, Zhong W, Zhang Y, Zhen C, Li Y, Jiang H, Meng T, Xu Y, Zhao G, Feng D
The EMBO journal 2022 Dec 15;41(24):e111173
The EMBO journal 2022 Dec 15;41(24):e111173
The mitochondrial UPR regulator ATF5 promotes intestinal barrier function via control of the satiety response.
Chamseddine D, Mahmud SA, Westfall AK, Castoe TA, Berg RE, Pellegrino MW
Cell reports 2022 Dec 13;41(11):111789
Cell reports 2022 Dec 13;41(11):111789
A novel mutation located in the intermembrane space domain of AFG3L2 causes dominant optic atrophy through decreasing the stability of the encoded protein.
Yang L, Jin X, Li Y, Guo Q, Yang M, You Y, Yao S, Zhang X, Wang Z, Lei B
Cell death discovery 2022 Aug 15;8(1):361
Cell death discovery 2022 Aug 15;8(1):361
Translation of MT-ATP6 pathogenic variants reveals distinct regulatory consequences from the co-translational quality control of mitochondrial protein synthesis.
Ng KY, Richter U, Jackson CB, Seneca S, Battersby BJ
Human molecular genetics 2022 Apr 22;31(8):1230-1241
Human molecular genetics 2022 Apr 22;31(8):1230-1241
A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.
Sun D, Niu Z, Zheng HX, Wu F, Jiang L, Han TQ, Wei Y, Wang J, Jin L
Cellular and molecular gastroenterology and hepatology 2021;11(4):1211-1226.e15
Cellular and molecular gastroenterology and hepatology 2021;11(4):1211-1226.e15
Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNA(His) mutation.
Gong S, Wang X, Meng F, Cui L, Yi Q, Zhao Q, Cang X, Cai Z, Mo JQ, Liang Y, Guan MX
The Journal of biological chemistry 2020 Jan 24;295(4):940-954
The Journal of biological chemistry 2020 Jan 24;295(4):940-954
Expanding the clinical and genetic heterogeneity of SPAX5.
Dosi C, Galatolo D, Rubegni A, Doccini S, Pasquariello R, Nesti C, Sicca F, Barghigiani M, Battini R, Tessa A, Santorelli FM
Annals of clinical and translational neurology 2020 Apr;7(4):595-601
Annals of clinical and translational neurology 2020 Apr;7(4):595-601
Sphingosine kinase 1 overexpression induces MFN2 fragmentation and alters mitochondrial matrix Ca(2+) handling in HeLa cells.
Pulli I, Löf C, Blom T, Asghar MY, Lassila T, Bäck N, Lin KL, Nyström JH, Kemppainen K, Toivola DM, Dufour E, Sanz A, Cooper HM, Parys JB, Törnquist K
Biochimica et biophysica acta. Molecular cell research 2019 Sep;1866(9):1475-1486
Biochimica et biophysica acta. Molecular cell research 2019 Sep;1866(9):1475-1486
Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.
Chen D, Zhang Z, Chen C, Yao S, Yang Q, Li F, He X, Ai C, Wang M, Guan MX
Nucleic acids research 2019 Jun 4;47(10):5341-5355
Nucleic acids research 2019 Jun 4;47(10):5341-5355
Mitochondrial stress response triggered by defects in protein synthesis quality control.
Richter U, Ng KY, Suomi F, Marttinen P, Turunen T, Jackson C, Suomalainen A, Vihinen H, Jokitalo E, Nyman TA, Isokallio MA, Stewart JB, Mancini C, Brusco A, Seneca S, Lombès A, Taylor RW, Battersby BJ
Life science alliance 2019 Feb;2(1)
Life science alliance 2019 Feb;2(1)
Fidelity of translation initiation is required for coordinated respiratory complex assembly.
Rudler DL, Hughes LA, Perks KL, Richman TR, Kuznetsova I, Ermer JA, Abudulai LN, Shearwood AJ, Viola HM, Hool LC, Siira SJ, Rackham O, Filipovska A
Science advances 2019 Dec;5(12):eaay2118
Science advances 2019 Dec;5(12):eaay2118
Sideroflexin 4 affects Fe-S cluster biogenesis, iron metabolism, mitochondrial respiration and heme biosynthetic enzymes.
Paul BT, Tesfay L, Winkler CR, Torti FM, Torti SV
Scientific reports 2019 Dec 23;9(1):19634
Scientific reports 2019 Dec 23;9(1):19634
Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.
Jian F, Chen D, Chen L, Yan C, Lu B, Zhu Y, Chen S, Shi A, Chan DC, Song Z
Cell reports 2018 Jun 5;23(10):2989-3005
Cell reports 2018 Jun 5;23(10):2989-3005
Adult-onset obesity is triggered by impaired mitochondrial gene expression.
Perks KL, Ferreira N, Richman TR, Ermer JA, Kuznetsova I, Shearwood AJ, Lee RG, Viola HM, Johnstone VPA, Matthews V, Hool LC, Rackham O, Filipovska A
Science advances 2017 Aug;3(8):e1700677
Science advances 2017 Aug;3(8):e1700677
Proteolytic control of the mitochondrial calcium uniporter complex.
Tsai CW, Wu Y, Pao PC, Phillips CB, Williams C, Miller C, Ranaghan M, Tsai MF
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 25;114(17):4388-4393
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 25;114(17):4388-4393
Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.
Richter U, Lahtinen T, Marttinen P, Suomi F, Battersby BJ
The Journal of cell biology 2015 Oct 26;211(2):373-89
The Journal of cell biology 2015 Oct 26;211(2):373-89
Novel MTND1 mutations cause isolated exercise intolerance, complex I deficiency and increased assembly factor expression.
Gorman GS, Blakely EL, Hornig-Do HT, Tuppen HA, Greaves LC, He L, Baker A, Falkous G, Newman J, Trenell MI, Lecky B, Petty RK, Turnbull DM, McFarland R, Taylor RW
Clinical science (London, England : 1979) 2015 Jun;128(12):895-904
Clinical science (London, England : 1979) 2015 Jun;128(12):895-904
Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.
Pfeffer G, Gorman GS, Griffin H, Kurzawa-Akanbi M, Blakely EL, Wilson I, Sitarz K, Moore D, Murphy JL, Alston CL, Pyle A, Coxhead J, Payne B, Gorrie GH, Longman C, Hadjivassiliou M, McConville J, Dick D, Imam I, Hilton D, Norwood F, Baker MR, Jaiser SR, Yu-Wai-Man P, Farrell M, McCarthy A, Lynch T, McFarland R, Schaefer AM, Turnbull DM, Horvath R, Taylor RW, Chinnery PF
Brain : a journal of neurology 2014 May;137(Pt 5):1323-36
Brain : a journal of neurology 2014 May;137(Pt 5):1323-36
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14631-1-AP(AFG3L2 antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 14631-1-AP(AFG3L2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 14631-1-AP(AFG3L2 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue