Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS3311 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS3311, RRID:AB_1662124
- Product name
- GÏ« t1 (M104) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 71-120 of Human GÏ« t1.
- Description
- Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein Ï«, ÉÇ and ÎÛ polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their Ï« subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of GÏ« subunits have been identified; these include Gs, Gi, Gq and GÏ« 12/13. The Gi class comprises all the known Ï« subunits that are susceptible to pertussis toxin modifications, including GÏ« i-1, GÏ« i-2, GÏ« i-3, GÏ« o, GÏ« t1, GÏ« t2, GÏ« z and GÏ« gust. In the well characterized visual system, photorhodopsin catalyzes the exchange of guanine nucleotides bound to the visual transducin GÏ« subunits (GÏ« t1 in rod cells and GÏ« t2 in cone cells).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of G¦Á t1 (M104) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysateLane3:PC12 whole cell lysate