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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- 66356-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRE Monoclonal Antibody (2E5A5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: PDPSALQNL ASWNPSNPEC LLLVVKELVQ QYHQFQCSRL RESSRLMFEY QTLLEEPQYG ENMEIYAGKK NNWTGEFSAR FLLKLPVDFS NIPTYLLKDV NEDPGEDVAL LSVSFEDTEA TQVYPKLYLS PRIEHALGGS SALHIPAFPG GGCLIDYVPQ VCHLLTNKVQ YVIQGYHKRR EYIAAFLSHF GTGVVEYDAE GFTKLTLLLM WKDFCFLVHI DLPLFFPRDQ PTLTFQSVYH FTNSGQLYSQ AQKNYPYSPR WDGNEMAKRA KAYFKTFVPQ FQEAAFANGK L (94-383 aa encoded by BC001251)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2E5A5
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66356-1-IG ( BRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 66356-1-Ig (BRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 66356-1-IG (BRE antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 66356-1-IG (BRE antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66356-1-IG ( BRE Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66356-1-IG ( BRE Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BRE antibody (Product # 66356-1-IG, Clone: 2E5A5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
