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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-21656 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, 293T, A431, C8D30, rat testis. Predicted reactivity: Mouse (99%), Rat (98%), Zebrafish (85%), Xenopus laevis (91%), Chicken (97%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRE was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BRE Polyclonal Antibody (Product # PA5-21656) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRE Polyclonal Antibody detects BRE protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract.10 % SDS-PAGE. BRE Polyclonal Antibody (Product # PA5-21656) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRE Polyclonal Antibody detects BRE protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract.10 % SDS-PAGE. BRE Polyclonal Antibody (Product # PA5-21656) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BRE in paraformaldehyde-fixed HeLa cells using a BRE polyclonal antibody (Product # PA5-21656) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRE antibody (Product # PA5-21656) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRE antibody immunoprecipitates BRE protein in IP experiments. IP Sample: 1,000 µg 293T whole cell lysate/extract A. Control with 2.5 µg of preimmune rabbit IgG B. Immunoprecipitation of BRE protein by 2.5 µg of BRE antibody (Product # PA5-21656) 10% SDS-PAGE The immunoprecipitated BRE protein was detected by BRE antibody (Product # PA5-21656) diluted at 1:1,000.
