TA809538

antibody from OriGene

Targeting: TRMT12 FLJ20772, Trm12, TYW2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA809538

Provider

OriGene

Product name

TRMT12 mouse monoclonal antibody,clone OTI5C9

Antibody type

Monoclonal

Description

TRMT12 mouse monoclonal antibody,clone OTI5C9

Host

Mouse

Conjugate

Unconjugated

Epitope

TRMT12

Isotype

IgG

Antibody clone number

5C9

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRMT12 (RC203982, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRMT12.(1:2000)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from HepG2 cell lines by using anti-TRMT12 monoclonal antibody .(1:500)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TRMT12 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809538)(1:500)

Validation comment

IHC