Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-59787 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- F1A alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NDRENYDIIK TYHYLYLAML ERFQDGDNIL EKEVLPPIHA YGNRTECRNP QELESIRQDR DALHMEGLIV RERILGADNI DVSHPIIY
- Concentration
- 0.9 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of F1A alpha was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR1054853_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of F1A alpha was performed by loading 60 µg of A549 Cas9 (Lane 1) and A549 F1A alpha KO (Lane 2) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with F1A alpha Polyclonal Antibody (Product # PA5-59787, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076) with the iBright™ FL1500 (Product # A44115). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to F1A alpha. Uncharacterized band was observed in both the samples at ~110 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of F1A alpha in human cell line A-431 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a F1A alpha Polyclonal Antibody (Product # PA5-59787).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of F1A alpha in human cell line A-431 using a F1A alpha Polyclonal Antibody (Product # PA5-59787) shows localization to nucleoplasm and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of F1A alpha in human testis using a F1A alpha Polyclonal Antibody (Product # PA5-59787) shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.