TA809024

antibody from OriGene

Targeting: NPPB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

TA809024

Provider

OriGene

Product name

NPPB mouse monoclonal antibody, clone OTI6F8 (formerly 6F8)

Antibody type

Monoclonal

Description

NPPB mouse monoclonal antibody, clone OTI6F8 (formerly 6F8)

Host

Mouse

Conjugate

Unconjugated

Epitope

NPPB

Isotype

IgG

Antibody clone number

OTI6F8

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPPB (RC206590, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPPB.(1:2000)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NPPB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809024)(1:150)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NPPB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809024)(1:150)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NPPB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120?‚°C for 3 min, TA809024)(1:150)

Validation comment

IHC

Other assay

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

NPPB(BNP/NT-ProBNP) Luminex ELISA with 8A9 Capture (TA809041) and 6F8 Detection (TA809024) Antibodies. Substrate used: Recombinant Human BNP/NT-ProBNP domain expressed in E.coli.

Validation comment

LMNX

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

NPPB(BNP/NT-ProBNP) Luminex ELISA with 17A6 Capture (TA809025) and 6F8 Detection (TA809024) Antibodies. Substrate used: Recombinant Human BNP/NT-ProBNP domain expressed in E.coli.

Validation comment

LMNX

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

NPPB(BNP/NT-ProBNP) Luminex ELISA with 3H2 Capture (TA809065) and 6F8 Detection (TA809024) Antibodies. Substrate used: Recombinant Human BNP/NT-ProBNP domain expressed in E.coli.

Validation comment

LMNX