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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- AM02178PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM02178PU-N, RRID:AB_1621418
- Product name
- anti Natriuretic peptides B (NT-proBNP 1-21)
- Antibody type
- Monoclonal
- Antigen
- Synthetic Human pro-BNP (aa 1-21) poly-Lysine conjugated
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 21-6-6
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry of proBNP staining in Paraffin section of Human tibial growth plate. Antigen retrieval was performed in 5 µg/ml proteinase K in 100mM Tris pH 8.0, 50mM EDTA for 10min at 37°C. The sections were incubated with AM02178PU (1/25) and detected using the biotin-streptavidin method. DAB was used as substrate. Sections were counterstained with hematoxylin. Image B: AM02178PU stains the late proliferative, prehypertrophic chondrocytes but not the resting and early proliferating cells. Image C: AM02178PU preincubated with proBNP (aa 1-21) does not stain the section. Marchini et al. (2007). Human Molecular Genetics 16(24): 3081-3087.
