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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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- Product number
- PA5-96084 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BNP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: Rat heart Immunogen sequence: SPKMVQGSGC FGRKMDR
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.56 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Nuclear-mitochondrial communication involving miR-181c plays an important role in cardiac dysfunction during obesity.
Roman B, Kaur P, Ashok D, Kohr M, Biswas R, O'Rourke B, Steenbergen C, Das S
Journal of molecular and cellular cardiology 2020 Jul;144:87-96
Journal of molecular and cellular cardiology 2020 Jul;144:87-96
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of BNP was performed in L929 cells using BNP Polyclonal Antibody (Product # PA5-96084) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of BNP in U2OS cells. Samples were incubated with BNP Polyclonal antibody (Product # PA5-96084) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of BNP in rat heart cells. Samples were incubated with BNP Polyclonal antibody (Product # PA5-96084) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BNP in paraffin-embedded mouse kidney. Samples were incubated with BNP Polyclonal antibody (Product # PA5-96084) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BNP in paraffin-embedded mouse heart. Samples were incubated with BNP Polyclonal antibody (Product # PA5-96084) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
