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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [3]
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- Product number
- CF809024 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPPB Monoclonal Antibody (OTI6F8), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI6F8
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPPB (RC206590, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NPPB. (1. Positive lysates LY400900 (100 µg) and LC400900 (20 µg) can be purchased separately from OriGene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NPPB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809024)(1:150).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NPPB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809024)(1:150).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil within the normal limits using anti-NPPB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809024)(1:150
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NPPB(BNP/NT-ProBNP) Luminex ELISA with 8A9 Capture (TA809041) and 6F8 Detection (TA809024) Antibodies. Substrate used: Recombinant Human BNP/NT-ProBNP domain expressed in E.coli.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NPPB(BNP/NT-ProBNP) Luminex ELISA with 3H2 Capture (TA809065) and 6F8 Detection (TA809024) Antibodies. Substrate used: Recombinant Human BNP/NT-ProBNP domain expressed in E.coli.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NPPB(BNP/NT-ProBNP) Luminex ELISA with 17A6 Capture (TA809025) and 6F8 Detection (TA809024) Antibodies. Substrate used: Recombinant Human BNP/NT-ProBNP domain expressed in E.coli.
