Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- Ab091484 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant BNP Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human BNP Antibody, Recombinant (R35-1H-4), could be used for WB and so on.Application:WB: 1/500-1/2000Protein RelevanceBrain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 50-102
- Antibody clone number
- R35-1H-4
- Vial size
- 100μl,10μl,1ml,50μl
- Concentration
- 0,95 mg/ml
- Storage
- Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant BNP Antibody (Ab091484) - Western Blot All lanes: Recombinant BNP Antibody (Ab091484) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 30 kDa Exposure time: 235.8 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant BNP Antibody (Ab091484) - Western Blot All lanes: Recombinant BNP Antibody (Ab091484) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 30 kDa Exposure time: 75.2 s