MA5-14830

antibody from Invitrogen Antibodies

Targeting: KIT C-Kit, CD117, PBT, SCFR

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA5-14830

Provider

Invitrogen Antibodies

Product name

Phospho-c-Kit (Tyr703) Monoclonal Antibody (G.813.2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

G.813.2

Vial size

100 µL

Concentration

50 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Phospho-c-Kit (Tyr703) Monoclonal Antibody (G.813.2) (Product # MA5-14830) and a 110 kDa band corresponding to Mast/stem cell growth factor receptor Kit was observed across cell line tested. Whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), IMR-23 treated with SCF (100 ng,30 min) (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).