Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- AM2137b - Provider product page
- Provider
- Abgent
- Product name
- KIT Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- KIT Antibody(Cat. #AM2137b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).
- Primary Ab dilution
- 1:100
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Immunofluorescent analysis of U251 cells, using KIT Antibody (Cat. #AM2137b). AM2137b was diluted at 1:100 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red)."
- Primary Ab dilution
- 1:100
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent confocal image of an SY5Y (SH-SY5Y; ATCC#CRL-2266) cell stained with AM2137b KIT antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AM2137b KIT primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 ¼M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Note the highly specific localization of the KIT immunosignal to the membranes, especially the plasma membrane. ibid. References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704]. Ibid."
- Primary Ab dilution
- 1:10~50