Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PB9258 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-c-Kit Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for C KIT/KIT detection. Host: Rabbit.Size: 100μg/vial. Tested applications: Flow Cytometry. Reactive species: Human. C KIT/KIT information: Molecular Weight: 109865 MW; Subcellular Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein; Tissue Specificity: Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references The Number of Interstitial Cells of Cajal Differs Among Different Subtypes of Achalasia and is Related to Patients' Prognosis.
Double sex and mab-3 related transcription factor 1 regulates differentiation and proliferation in dairy goat male germline stem cells.
Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis.
Impact of the alterations in the interstitial cells of Cajal on intestinal motility in post-infection irritable bowel syndrome.
Wnt1 inhibits hydrogen peroxide-induced apoptosis in mouse cardiac stem cells.
Isolation, culture and characterization of cardiac progenitor cells derived from human embryonic heart tubes.
Activation of protein kinases A and C promoted proliferation of chicken primordial germ cells.
Stimulating effects of androgen on proliferation of cultured ovarian germ cells through androgenic and estrogenic actions in embryonic chickens.
Effects of follicle-stimulating hormone and androgen on proliferation of cultured testicular germ cells of embryonic chickens.
Effects of follicle-stimulating hormone and 17beta-estradiol on proliferation of chicken embryonic ovarian germ cells in culture.
Chen S, Zhang M, Liang M, Tan N, Cui Y, Wang J, Xin X, Ye Z, Zhuang Q, Xiao Y
Clinical and translational gastroenterology 2021 Aug 24;12(8):e00388
Clinical and translational gastroenterology 2021 Aug 24;12(8):e00388
Double sex and mab-3 related transcription factor 1 regulates differentiation and proliferation in dairy goat male germline stem cells.
Wei Y, Cai S, Ma F, Zhang Y, Zhou Z, Xu S, Zhang M, Peng S, Hua J
Journal of cellular physiology 2018 Mar;233(3):2537-2548
Journal of cellular physiology 2018 Mar;233(3):2537-2548
Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis.
Yang J, Chi C, Liu Z, Yang G, Shen ZJ, Yang XJ
Journal of cellular and molecular medicine 2015 Jul;19(7):1720-8
Journal of cellular and molecular medicine 2015 Jul;19(7):1720-8
Impact of the alterations in the interstitial cells of Cajal on intestinal motility in post-infection irritable bowel syndrome.
Yang B, Zhou XC, Lan C
Molecular medicine reports 2015 Apr;11(4):2735-40
Molecular medicine reports 2015 Apr;11(4):2735-40
Wnt1 inhibits hydrogen peroxide-induced apoptosis in mouse cardiac stem cells.
Liu J, Wang Y, Du W, Liu W, Liu F, Zhang L, Zhang M, Hou M, Liu K, Zhang S, Yu B
PloS one 2013;8(3):e58883
PloS one 2013;8(3):e58883
Isolation, culture and characterization of cardiac progenitor cells derived from human embryonic heart tubes.
Zhang X, Zhang CS, Liu YC, Yang XQ, Xiong SH, Wen Y, Jiang EP, Li R, Zhang ZY, Liu F, Ye Y
Cells, tissues, organs 2009;190(4):194-208
Cells, tissues, organs 2009;190(4):194-208
Activation of protein kinases A and C promoted proliferation of chicken primordial germ cells.
Tang X, Zhang C
Animal reproduction science 2007 Oct;101(3-4):295-303
Animal reproduction science 2007 Oct;101(3-4):295-303
Stimulating effects of androgen on proliferation of cultured ovarian germ cells through androgenic and estrogenic actions in embryonic chickens.
Liu H, Zhang C, Tang X, Zeng W, Mi Y
Domestic animal endocrinology 2005 May;28(4):451-62
Domestic animal endocrinology 2005 May;28(4):451-62
Effects of follicle-stimulating hormone and androgen on proliferation of cultured testicular germ cells of embryonic chickens.
Mi Y, Zhang C, Xie M, Zeng W
General and comparative endocrinology 2004 Sep 15;138(3):237-46
General and comparative endocrinology 2004 Sep 15;138(3):237-46
Effects of follicle-stimulating hormone and 17beta-estradiol on proliferation of chicken embryonic ovarian germ cells in culture.
Xie M, Zhang C, Zeng W, Mi Y
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2004 Dec;139(4):521-6
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2004 Dec;139(4):521-6
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of C-Kit using anti-C-Kit antibody (PB9258). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinan Human C-Kit Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Kit antigen affinity purified polyclonal antibody (Catalog # PB9258) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C-Kit at approximately 49KD. The expected band size for C-Kit is at 49KD.
- Additional image